RESUMO
OBJECTIVE: Comparing modified Ziehl-Neelsen (ZNm) and modified Safranin (Sm) staining for detecting C. cayetanensis oocysts in stool samples. METHODS: The sample to be analysed consisted of 100 stool samples which had been previously evaluated by direct microscopic examination and concentration. Each sample was then ZNm and Sm stained. Microscope examination was used as gold standard for comparing both techniques by statistical analysis. RESULTS: There was 95% sensitivity and 90% specificity for ZNm and 98% sensitivity and 100% specificity for Sm. The Kappa index was 0.93, signifying a very good degree of agreement between the two techniques. CONCLUSIONS: The use of either of the two stains for diagnosing C. cayetanensis can be recommended due to the high sensitivity and specificity for Zm and Sm found in this study and to the high degree of agreement between them.
Assuntos
Corantes , Cyclospora/isolamento & purificação , Fezes/parasitologia , Fenazinas , Coloração e Rotulagem/métodos , Animais , HumanosRESUMO
Objetivo Comparar las coloraciones Ziehl-Neelsen modificada (ZNm) y Safranina modificada (Sm) en la detección de ooquistes de C. cayetanensis en muestras de materia fecal . Metodología Se analizaron 100 muestras de materia fecal que fueron evaluadas previamente por coprológico directo y por concentración, posteriormente se realizaron las coloraciones ZNm y Sm para cada una de las muestras. Para la comparación de las dos técnicas se hicieron análisis estadísticos tomando como prueba oro el examen directo. Resultados Se encontró una sensibilidad y especificidad del 95 por ciento y 98 por ciento para ZNm y del 90 por ciento y 100 por ciento para Sm respectivamente. El índice Kappa fue de 0,93, correspondiendo a un muy buen grado de concordancia entre las dos coloraciones. Conclusiones Debido a la alta sensibilidad y especificidad encontradas en este estudio para Zm y Sm y al alto grado de concordancia entre ellas, cualquiera de las dos coloraciones puede ser utilizada para el diagnóstico de rutina de C. cayetanensis.
Objective Comparing modified Ziehl-Neelsen (ZNm) and modified Safranin (Sm) staining for detecting C. cayetanensis oocysts in stool samples. Methods The sample to be analysed consisted of 100 stool samples which had been previously evaluated by direct microscopic examination and concentration. Each sample was then ZNm and Sm stained. Microscope examination was used as gold standard for comparing both techniques by statistical analysis. Results There was 95 percent sensitivity and 90 percent specificity for ZNm and 98 percent sensitivity and 100 percent specificity for Sm. The Kappa index was 0,93, signifying a very good degree of agreement between the two techniques. Conclusions The use of either of the two stains for diagnosing C. cayetanensis can be recommended due to the high sensitivity and specificity for Zm and Sm found in this study and to the high degree of agreement between them.
